Evaluation of Ros Induced Genetic Aberration in Benzene Intoxicated Rat Bone Marrow

Here we aimed to observe the dose dependent genotoxic potential of benzene which may trigger the leukomogenic activity in bone marrow of rat. We used Wistar rat as a model and benzene was administrated orally at the doses of 50mg/ml/ day and 100mg/ml / day for 90 days which exhibited with definite signs of genotoxicity which may initiate carcinogenesis. Cellular stress of bone marrow was evaluated through various ROS parameter which was resulted into significant decrease in the level of antioxidative enzyme activity. There was significantly increased frequency of micronuclei in bone marrow of rat especially in polychromatic erythrocyte and the ratio of polychromatic and normochromatic erythrocyte was depleted at the significant level. Structural chromosomal aberration was observed significantly higher at 100mg/ml exposure of rat. The extent of DNA damage was evaluated through comet assay. A significant increase in the tail length of comet was recorded in the benzene exposed rats. Furthermore, the analysis of DNA ladder assay and cell morphology study of bone marrow cell was revealed the apparent hike of apoptotic cell death due to benzene intoxication. Present finding indicate that benzene exposure develops cancer pathogenesis in rat bone marrow by modulating the oxidative stress parameters and apoptosis